Isolation of Full-Length cDNA Clones Using SMARTTM cDNA and a Biotin-Streptavidin Bead System
نویسندگان
چکیده
منابع مشابه
Large-scale RT-PCR recovery of full-length cDNA clones.
Pseudogenes, alternative transcripts, noncoding RNA, and polymorphisms each add extensive complexity to the mammalian transcriptome and confound estimation of the total number of genes. Despite advanced algorithms for gene prediction and several large-scale efforts to obtain cDNA clones for all human open reading frames (ORFs), no single collection is complete. To enhance this effort, we have d...
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Please be advised that this information was generated on 2017-04-04 and may be subject to change.
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Most cDNA library screening procedures do not distinguish between full-length and incomplete clones and therefore may yield incomplete cDNA fragments. Thus, there is a widespread need for a method allowing the efficient selection of full-length clones. I present a rapid, PCR-based method that allows the simultaneous screening of > 10(6) cDNAs. The longest cDNA is identified in the first step so...
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Receptors for the Fc region of IgG (FcyR)' are expressed in a number of hematopoietic cell types and play important roles in several immunological processes such as phagocytosis of opsonized particulate antigens (1), clearance of immune complexes (2, 3), antibody-dependent cellular cytotoxicity (4, 5), signalling the production of inflammatory mediators (6), and regulation of Ig synthesis (7, 8...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00293bm08